mouse pinp elisa kit  (Cusabio)

Journal: Frontiers in Endocrinology

Article Title: Anoctamin 5 mutation leads to abnormal bone homeostasis of GDD by regulating AMPK-dependent glucose metabolism

doi: 10.3389/fendo.2026.1703491

Figure Lengend Snippet: AMPK inhibitor regulates osteogenesis and osteoclastogenesis of GDD in vivo . 12-week-old male mice were used to observe bone phenotype. (A) Representative photomicrographs and quantitative immuno-positive area analysis of OCN in tibia (bar=100 μm); (B–D) ELISA analysis of serum level of ALP, PINP (B) , OPG, OPG/RANKL (C) , and CTX (D, E) Representative images of TRAP staining and quantitative analysis of the osteoclast number (Oc.N)/perimeter of bone (B.Pm) in the germinal center of the tibia (bar=100 μm). Data are presented as mean ± SEM. Statistic significances are determined by one-way ANOVAs with Dunnett’s multiple comparison tests, with ns: no significance, *P < 0.05, **P < 0.01.

Article Snippet: Procollagen I N-terminal propeptide (PINP) and type I collagen cross-linked C-telopeptide (CTX) in the serum were measured by using mouse PINP ELISA Kit (Elabscience, E-EL-M0233) and mouse β-CTx ELISA Kit (Elabscience, E-EL-M0372) following the manufacturer’s protocol.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Staining, Comparison

Journal: Nature Communications

Article Title: RKIP regulates bone marrow macrophage differentiation to mediate osteoclastogenesis and H-type vessel formation

doi: 10.1038/s41467-025-62972-8

Figure Lengend Snippet: A Micro-CT and three-dimensional (3D) reconstruction images of the distal femur of 3-month-old male RKIP +/+ or RKIP -/- mice are shown. B Quantification of bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular separation (Tb.Sp), trabecular thickness (Tb.Th), and cortical thickness (Ct.Th) ( n = 5). C H&E staining and TRAP staining of 3-month-old male RKIP +/+ or RKIP -/- mice femurs sections. D Quantification of osteoclast number per bone surface (N.Oc/BS) and osteoclast surface per bone surface (Oc.S/BS) ( n = 6). E Calcein double staining and Von Kossa staining of 3-month-old male RKIP +/+ or RKIP -/- mice femurs sections. F Quantification of mineral apposition rate (MAR) and bone formation rate per bone surface (BFR/BS) ( n = 6). G OCN (green) and DAPI (blue) immunofluorescence of 3-month-old male RKIP +/+ or RKIP -/- mice femurs sections; The arrow indicates the cells which express OCN. H Quantification of OCN + cell number per bone perimeter (N.OCN + /B.Pm) ( n = 6). I Serum C-terminal telopeptide of type I collagen (CTX-1) and Procollagen I N-Terminal Propeptide (PINP) concentration measured by ELISA from RKIP +/+ or RKIP -/- male mice ( n = 6). Data are presented as mean ± SD. * P < 0.05, ** P < 0.01 for a comparison with the control group or as indicated. n value means the number of repetitions in each independent experiment.

Article Snippet: Locostatin (#HY-W013411A, MCE, USA), Didymin (#HY-N2068, MCE, USA), recombinant mouse M-CSF (#CB34, Novoprotein, China) and RANKL (#462-TEC, R&D, USA), recombinant human M-CSF (#C417, Novoprotein, China) and RANKL (#CK63, Novoprotein, China), TRAP staining kits (#387A-1KT, Sigma-Aldrich, USA), Mouse CTX-1 ELISA Kit (#CSB-E12782m, CUSABIO, China), Mouse PINP ELISA Kit (#CSB-E12775m, CUSABIO, China) were used.

Techniques: Micro-CT, Staining, Double Staining, Immunofluorescence, Concentration Assay, Enzyme-linked Immunosorbent Assay, Comparison, Control

Journal: Bone Research

Article Title: Bone marrow adipogenic lineage precursors are the major regulator of bone resorption in adult mice

doi: 10.1038/s41413-025-00405-4

Figure Lengend Snippet: Depletion of RANKL in MALPs increases long bone trabecular bone mass in adult mice by suppressing bone resorption. a qRT-PCR analysis of Rankl mRNA in bone marrow and cortical bone from WT and RANKL iCKO mice at 4 weeks after Tam injection. Mice received Tam at 3 months of age. n = 3 mice/group. b ELISA analysis of RANKL in bone marrow from WT and RANKL iCKO mice at 2 weeks after Tam injection. Mice received Tam at 3 months of age. n = 3 mice/group. c 3D microCT reconstruction of whole femurs from WT and iCKO mice at 1 month after Tam injection. Scale bar = 1 mm. d 3D microCT reconstruction reveals a drastic increase of femoral trabecular bone. Scale bar = 200 µm. e MicroCT measurement of trabecular bone structural parameters. BV/TV bone volume fraction, Tb.N trabecular number, Tb.Th trabecular thickness, Tb.Sp trabecular separation. f Representative TRAP staining images show TRAP+ osteoclast (arrows) at different skeletal sites: secondary spongiosa (SS), chondro-osseous junction (COJ), and endosteal surface (Endo.S). Scale bar = 50 μm. g Quantification of osteoclast surface (Oc.S) at 3 skeletal sites. BS bone surface, L COJ length. h Representative Osterix staining of trabecular bone from WT and RANKL iCKO femurs. Scale bar = 50 μm. i Quantification of osteoblast surface (OB.S). j Representative double labeling of trabecular bone from WT and iCKO femurs. Scale bar = 20 μm. k Bone formation activity is quantified. MAR mineral apposition rate, MS mineralizing surface, BFR bone formation rate. l Serum ELISA analysis of bone resorption marker (CTX-1) and formation marker (P1NP) in WT and iCKO mice. * P < 0.05; ** P < 0.01; *** P < 0.001 vs WT , n = 5–6 mice/group

Article Snippet: Sera were collected during mouse euthanization for measuring bone turnover markers, collagen type I C-telopeptide degradation products (mouse CTX-I ELISA Kit, MyBioSource), N-terminal propeptide of type I procollagen (ImmunotagTM Mouse PINP ELISA Kit, G-Bioscience) according to the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Injection, Enzyme-linked Immunosorbent Assay, Staining, Labeling, Activity Assay, Marker

Journal: Bone Research

Article Title: Bone marrow adipogenic lineage precursors are the major regulator of bone resorption in adult mice

doi: 10.1038/s41413-025-00405-4

Figure Lengend Snippet: RANKL deficiency in MALPs protects adult female mice from ovariectomy-induced trabecular bone loss. a 3D microCT reconstruction of femoral trabecular bone from WT and RANKL iCKO mice at 6 weeks post OVX surgery. Mice received Tam injections at 3 months of age right before the surgery. Scale bar = 200 µm. b MicroCT measurement of trabecular bone structural parameters. c Representative TRAP staining images of trabecular bone from WT and RANKL iCKO femurs show TRAP + osteoclasts (arrows). Scale bar = 50 μm. d Quantification of osteoclast surface (Oc.S). e Representative Osterix staining of trabecular bone from WT and RANKL iCKO femurs. Scale bar = 50 μm. f Quantification of osteoblast surface (OB.S). g Bone formation activity is quantified. h Serum ELISA analysis of bone resorption marker (CTX-1) and formation marker (P1NP) in WT and iCKO mice. i Representative H&E staining of trabecular bone from WT and RANKL iCKO femurs. Scale bar = 50 μm. j Quantification of the percentage of adipocyte area within bone marrow and adipocyte size. # P < 0.05; ## P < 0.01; ### P < 0.001 OVX vs Sham; * P < 0.05; ** P < 0.01; *** P < 0.001 iCKO vs WT , n = 5–6 mice/group

Article Snippet: Sera were collected during mouse euthanization for measuring bone turnover markers, collagen type I C-telopeptide degradation products (mouse CTX-I ELISA Kit, MyBioSource), N-terminal propeptide of type I procollagen (ImmunotagTM Mouse PINP ELISA Kit, G-Bioscience) according to the manufacturer’s instructions.

Techniques: Staining, Activity Assay, Enzyme-linked Immunosorbent Assay, Marker

Journal: Bone Research

Article Title: Bone marrow adipogenic lineage precursors are the major regulator of bone resorption in adult mice

doi: 10.1038/s41413-025-00405-4

Figure Lengend Snippet: Depleting RANKL in MALPs in osteoporotic mice restores trabecular bone mass. a 3D microCT reconstruction of femoral trabecular bone from WT and RANKL iCKO mice at 10 weeks post OVX surgery. Mice received the surgery at 3 months of age and vehicle or Tam injections 6 weeks later. Scale bar = 200 µm. b MicroCT measurement of trabecular bone structural parameters. c Representative TRAP staining images of femoral trabecular bone from WT and RANKL iCKO mice with vehicle or Tam injections show TRAP + osteoclasts (arrows). Scale bar = 50 μm. d Quantification of osteoclast surface (Oc.S). e Representative Osterix staining of femoral trabecular bone from WT and RANKL iCKO mice with vehicle or Tam injections. Scale bar = 50 μm. f Quantification of osteoblast surface (OB.S). g Bone formation activity is quantified. h Serum ELISA analysis of bone resorption marker (CTX-1) and formation marker (P1NP) in WT and iCKO mice with vehicle or Tam injections. # P < 0.05; ## P < 0.01; ## P < 0.001 Tam vs Veh; * P < 0.05; ** P < 0.01; *** P < 0.001 iCKO vs WT , n = 5–6 mice/group

Article Snippet: Sera were collected during mouse euthanization for measuring bone turnover markers, collagen type I C-telopeptide degradation products (mouse CTX-I ELISA Kit, MyBioSource), N-terminal propeptide of type I procollagen (ImmunotagTM Mouse PINP ELISA Kit, G-Bioscience) according to the manufacturer’s instructions.

Techniques: Staining, Activity Assay, Enzyme-linked Immunosorbent Assay, Marker

Journal: BMB Reports

Article Title: Wnt5a exacerbates pathological bone features and trabecular bone loss in curdlan-injected SKG mice via osteoclast activation

doi: 10.5483/BMBRep.2024-0155

Figure Lengend Snippet: Treatment with Wnt5a facilitated an increase in osteoclast activity and trabecular bone loss of curdlan-injected SKG mice in vivo . The Vehicle and Wnt5a-treated groups were analyzed using the following: (A) representative microCT images of femurs from each group; (B) quantitative microCT data, including trabecular volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation; (C) ELISA measurements of PINP (bone formation marker) and CTX-1 (bone resorption marker); and (D) representative H&E and TRAP staining images of femurs, alongside quantification of TRAP-positive areas. Osteoclast differentiation of bone marrow–derived OCPs from SKG mice was assessed via (E) TRAP and F-actin staining and (F) osteoclast-differentiation markers like Itgb3, Dcstamp, Nfatc1, Ctsk, and Acp5 . Values are expressed as mean ± SEM. *P < 0.05, **P < 0.01 by Mann–Whitney U test.

Article Snippet: ELISA assay for PINP (CSB-E12775m; CUSABIO), and CTX-1 (CSB-E12782m; CUSABIO) were carried out following the manufacturer’s instructions.

Techniques: Activity Assay, Injection, In Vivo, Enzyme-linked Immunosorbent Assay, Marker, Staining, Derivative Assay, MANN-WHITNEY